Abstract
Lysophosphatidylcholine (LPC) has been shown to have many physological functions such as the contraction of smooth muscle cells, secretion of a growth factor from endotherial cells and the chemoattraction of T-cells. Lysophospholipids derived from soy bean in this study was noted to enhance the production of glycosaminoglycan (GAG), particularly hyaluronic acid, in a confluent culture of normal human dermal fibroblasts in Dulbecco's modified Eagle medium containing 0.5% FCS.
The pretreatment of the lysophospholipids by phospholipase B resulted in less stimulation of GAG production. Glycerol, glycerophosphate, glycerophosphorylcholine and all fatty acids failed to stimulate GAG production. LPC from soy bean enhanced GAG production, but not those from egg yolk or bovine liver. LPCs bearing acyl-residues between any two carbons from 6 to 18, C12 : 0-LPC, C14 : 1-LPC and C18 : 3-LPC significantly enhanced GAG production. The separate ad-dition of any LPC component in soy bean to the medium did not result in greater GAG production, but when all components were added together each at the same molar ratio as soy bean, GAG synthesis was stimulated.
