Abstract
Determination was made of phytosterol composition in the seeds of Echinochloa crus-galli and Setaria italica Beauv. The phytosterol fractions were extracted, purified and analyzed by GC-MS following trimethylsilylation. In the seeds of E. crus-galli, cholesterol, 24-methylenecholesterol, campesterol, campestanol, stigmasterol, sitosterol, sitostanol and isofucosterol were identified. In the seeds of S. italica, campesterol, campestanol, stigmasterol, Δ22-stigmasterol, sitosterol, sitostanol, and Δ7-stigmastenol were found present. Phytosterol compositions in the two species of seeds of E. crus-galli and S. italica were clarified. It is interesting that in the seeds of S. italica, sitostanol was found to account for 26% of the total sterols.