1999 Volume 48 Issue 10 Pages 1169-1176,1203
Docosahexaenoic acid ethyl ester (DHA-E) could be refined by a pilot-scale Supercritical Fluid Chromatography (SFC) using silver-loaded spherical clay material [SC (Ag)] in a new chromatographic stationary phase. High purity DHA-E (99%) could thus be recovered to a greater degree by using the fish oil with high DHA-E content, and by enlarging the preparative column. This DHA-E was found to have reagent grade purity, and purification was not attended with deterioration. The time required for purification could be shortened by changing the purity of DHA-E from 99% to 95%, and more DHA-E per day of higher purity could thus be obtained by this new method.
