Abstract
During the autoxidation of methyl linolenate, a group of degradation products capable of inhibiting β-trypsin activity were generated along with the accelerating substances, as also noted in a previous study. Chemical modification of the functional groups indicated the carbonyl and hydroxyl groups of the inhibitory degradation products and ε-amino group (s) of trypsin to possibly be essential for the inhibition, but hydroperoxide not necessarily involved in inhibitory activity. The inhibitory degradation products inhibited α-chymotrypsin and lysozyme activity and thus may be inhibitors of various enzymes. There is thus the possibility that enzyme inhibitors and accelerators are generated spontaneously during the autoxidation of unsaturated lipids.
