Abstract
In the process of screening of lipase producing bacteria from the soil of vegetable oil industries, a particular gram negative, rod shaped lipase synthesising bacterial strain, Burkholderia cepacia SS-16 was obtained. The maximum lipase activity of the bacteria was obtained after 72h of growth. The optimal temperature at pH 6.5-7.0 and the optimal pH at a temperature of 35°C were 60°C and 7.0, respectively. The lipase was stable to a temperature of 70°C and a pH of 8.0. The bacterial lipase was most stable in water miscible solvent isopropanol and cleaved triacylglycerol in a random manner.
