Cell viability and physicochemical effects of different concentrations of bismuth trioxide in a mineral trioxide aggregate cement

Karla Padrón-Alvarado; Laura A. García-Mendoza; Juana P. Ramírez-Ortega; Jacqueline A. Rodríguez-Chavéz; María D. L. A. Moyaho-Bernal; Juan A. Arreguín-Cano; Lia A. Hoz-Rodríguez; Ana Wintergerst; Jesús A. Arenas-Alatorre; Abigailt Flores-Ledesma

doi:10.2334/josnusd.22-0139

Original Article

Cell viability and physicochemical effects of different concentrations of bismuth trioxide in a mineral trioxide aggregate cement

Karla Padrón-Alvarado, Laura A. García-Mendoza, Juana P. Ramírez-Ortega, Jacqueline A. Rodríguez-Chavéz, María D. L. A. Moyaho-Bernal, Juan A. Arreguín-Cano, Lia A. Hoz-Rodríguez, Ana Wintergerst, Jesús A. Arenas-Alatorre, Abigailt Flores-Ledesma

Author information

Karla Padrón-Alvarado
Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of México
Laura A. García-Mendoza
Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of México
Juana P. Ramírez-Ortega
Dental Materials and Biomaterials Research Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of México
Jacqueline A. Rodríguez-Chavéz
Department of Integral Dental Clinics, University Center for Health Sciences, University of Guadalajara
María D. L. A. Moyaho-Bernal
Dental Materials and Biomaterials Laboratory Faculty of Stomatology, Meritorious Autonomous University of Puebla
Juan A. Arreguín-Cano
Health of the State of Guerrero
Lia A. Hoz-Rodríguez
Periodontal Biology and Mineralized Tissue Laboratory, Postgraduate Studies and Research Division, School of Dentistry, National Autonomous University of México
Ana Wintergerst
Division of Postgraduate Studies and Research, School of Dentistry, National Autonomous University of México
Jesús A. Arenas-Alatorre
Central Microscopy Laboratory, Institute of Physics, National Autonomous University of México
Abigailt Flores-Ledesma
Dental Materials and Biomaterials Laboratory Faculty of Stomatology, Meritorious Autonomous University of Puebla

Keywords: bismuth trioxide, cell viability, mineral trioxide aggregate, physical properties, radiopacity

JOURNAL OPEN ACCESS

2023 Volume 65 Issue 1 Pages 10-14

DOI https://doi.org/10.2334/josnusd.22-0139

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Abstract

Purpose: To evaluate the effect of three concentrations of bismuth trioxide (Bi₂O₃) on the biological and physicochemical properties of an experimental mineral trioxide aggregate-type (MTA-type) cement at different time points.

Methods: Three experimental groups with white Portland cement containing 15, 20, or 25 wt% of Bi₂O₃ were assessed. Cellular proliferation in human periodontal ligament fibroblasts was evaluated with an MTT assay. Radiopacity, dimensional stability, pH, and compressive strength were evaluated at different time points.

Results: Bismuth trioxide induced cell proliferation in the Bi15 and Bi25 groups in a time-dependent manner; pH was similar in all groups. Compressive strength was associated with time and bismuth concentration. Bi25 had significantly contracted at day 7 and expanded at day 14 (ANOVA P < 0.05, post hoc Tukey test P < 0.05).

Conclusion: A higher Bi₂O₃ concentration had a negative effect on the physical properties of the cement at all time points.

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