The color reaction of α-cholestanol with acyloxysulfonium salt solutions prepared by several sulfoxides and acetyl chloride is reported. Optimal conditions for the reaction which could be employed were 1.25 mmoles acyloxysulfonium salt solution prepared by dimethyl sulfoxide and acetyl ckloride per mmole of α-cholestanol reacted at 70°C. The solution prepared by methyl methylthiomethyl sulfoxide and acetyl chloride can be used in place of dimethyl sulfoxide for the method of detection of α-cholestanol. The other sulfoxides, tetramethylene sulfoxide and diphenyl sulfoxide, were of no use for the method the authors employed.
