Protocol for TMT-labeling of peptides in quantitative proteomics

Abstract

Isobaric tags, such as Tandem Mass Tag™ (TMT; Thermo Fisher Scientific), TMTpro™ (Thermo Fisher Scientific), or iTRAQ^® (Sciex), allow reproducible and high-throughput peptide/protein quantification in mass spectrometry-based proteomics. Herein, we introduce a practical protocol for TMT-labeling of peptide samples, with troubleshooting for possible problems and examples of important parameters in measurement by mass spectrometry.