Dataset for evaluation of the lauryl maltose neopentyl glycol assisted sample preparation method

Ryo Konno; Masaki Ishikawa; Daisuke Nakajima; Yusuke Endo; Osamu Ohara; Yusuke Kawashima

doi:10.14889/jpdm.2024.0007

Ryo Konno, Masaki Ishikawa, Daisuke Nakajima, Yusuke Endo, Osamu Ohara, Yusuke Kawashima

Author information

Ryo Konno
https://orcid.org/0000-0002-4375-1133
Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan
Masaki Ishikawa
https://orcid.org/0000-0002-3101-6713
Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan
Daisuke Nakajima
https://orcid.org/0000-0003-3310-3762
Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan
Yusuke Endo
https://orcid.org/0000-0002-7223-1938
Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, 2-6-7 Kazusa Kamatari, Kisarazu, Chiba, 292-0818, Japan
Department of Omics Medicine, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba 260-8670 Japan
Osamu Ohara
https://orcid.org/0000-0002-3328-9571
Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan
Yusuke Kawashima Corresponding author
https://orcid.org/0000-0002-9779-8199
Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan

Keywords: LMNG, DMNG, LASP, scpLASP, single cell proteomics, EVOSEP ONE

JOURNAL OPEN ACCESS
Supplementary material

2024 Volume 6 Pages 7-

DOI https://doi.org/10.14889/jpdm.2024.0007

Details

Abstract

We have developed a lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method for reducing protein and peptide loss. LMNG was removed simply via reversed phase solid-phase extraction. LASP method was applied to the low-input SP3 method and EVOSEP ONE combined with LC-MS/MS. Furthermore, we have established sample preparation method for single cell proteomics based on the LASP method (scpLASP).

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