Data for proteomic analysis of the chaperone-like nuclear VCP-like 2 (NVL2)-associated proteins

Abstract

Nuclear p97/Valosin-containing protein (VCP)-like 2 (NVL2) is a nucleolus-localized chaperone-like protein that belongs to the type II class of ATPases associated with diverse cellular activities (AAA) family. In the nucleolus, NVL2 is involved in the maturation of pre-60S particles in an ATP hydrolysis-dependent manner by associating with transactivating factors involved in ribosome biogenesis or the RNA degradation/processing complex, RNA exosome. To perform a comparative analysis of NVL2-associated protein complexes based on their ATP hydrolysis activity, NVL2 (wild-type or ATP hydrolysis-deficient)-associated protein complexes were analyzed. FLAG-tagged NVL2 (wild-type or ATP hydrolysis-deficient mutant, E365Q/E682Q) inducible Flp-In T-REx 293 cell lines were established, and affinity-purifications of the NVL2-associated protein complexes using FLAG tag were performed. NVL2 (wild-type or ATP hydrolysis-deficient)-associated proteins were identified by IP-MS with data dependent acquisition method.