Data for comparative proteomic analysis of Calu-3 cells infected with SARS-CoV-2 in the absence and presence of calpeptin

Fumiko Shinkai-Ouchi; Mayumi Shindo; Motohiko Ogawa; Naoko Doi; Masayoshi Fukasawa; Yasuko Ono

doi:10.14889/jpdm.2025.0003

Fumiko Shinkai-Ouchi , Mayumi Shindo, Motohiko Ogawa, Naoko Doi, Masayoshi Fukasawa, Yasuko Ono

Author information

Fumiko Shinkai-Ouchi Corresponding author
https://orcid.org/0000-0002-0095-941X
Calpain project, Department of Basic Medical Sciences, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Mayumi Shindo
Protein analysis, Center for Basic Technology Research, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Motohiko Ogawa
https://orcid.org/0000-0002-5516-0998
Department of Bacteriology 1, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan
Naoko Doi
Calpain project, Department of Basic Medical Sciences, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Masayoshi Fukasawa
https://orcid.org/0000-0002-0702-0691
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan
Yasuko Ono Corresponding author
https://orcid.org/0000-0001-9588-0087
Calpain project, Department of Basic Medical Sciences, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

Keywords: SARS-CoV-2, calpeptin, calpain, host-response to infection

JOURNAL OPEN ACCESS
Supplementary material

2025 Volume 7 Pages 3-

DOI https://doi.org/10.14889/jpdm.2025.0003

Details

Abstract

We analyzed the alteration of the host cell proteome upon the SARS-CoV-2 infection in the absence and presence of cysteine protease inhibitor, calpeptin, which is effective to virus protease Mpro and host cysteine proteases including L-cathepsin and calpain. The present data was obtained using human lung adenocarcinoma cell line Calu-3 cells (HTB-55, ATCC^®) and SARS-CoV-2 strain JPN/TY/WK-521 (GISAID_ID: EPI_ISL_408667).

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