Matrix effect of spinach on agricultural chemical residue analysis by a commercially available ELISA kit

Shoko Amano; Shinobu Suga; Sadanori Sawano

doi:10.1584/jpestics.32.300

Note (with abstract in English)

Matrix effect of spinach on agricultural chemical residue analysis by a commercially available ELISA kit

Shoko Amano, Shinobu Suga, Sadanori Sawano

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Keywords: ELISA kit, spinach, matrix interference, ultrafiltration

JOURNAL FREE ACCESS

2007 Volume 32 Issue 3 Pages 300-304

DOI https://doi.org/10.1584/jpestics.32.300

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Abstract

The matrix effect of spinach on residue analysis by commercially available ELISA kits was significant and different by every kit used. In particular, a serious influence was observed with the kit for imidacloprid and fenitrothion measurement. The substances that caused the matrix effect were removed with ultrafiltration (UF10000). It was conjectured that these substances is hydrophobic particles in the suspension.

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