Demonstration of Glucose Gatabolizing Inductive Enzymes in Xanthomonas Bacteria by Protein Synthesis Inhibitors

Pharmacological Studies on Control of the Xanthomonas Bacteria Diseases (Part 2)

Abstract

The earlier comparative study on glucose catabolism of three pathovars of Xanthomonas species in this laboratory revealed that a set of glucose catabolizing enzymes was specifically induced in the presence of glucose when Xanthomonas campestris pv, oryzae was precultured on succinate as a sole carbon source. In this study, it was further demonstrated that the determination of O₂ uptake and enzymic activities of various inducible enzymes in the glucose catabolism with regard to Xanthomonas campestris pv. oryzae showed the repression of the activities of 6-phosphofructokinase (EC 2. 7. 1. 11), phosphopyruvate hydratase (EC 4. 2. 1. 11) and phosphogluconate dehydrogenase (EC 1. 1. 1. 43) in the presence of sub-lethal concentration of streptomycin (100ppm) or chloramphenicol (100ppm) excluding the cases of other three Xanthomonas campestris pathovars.