Method to Determination of Total Residues of the Insecticide Acetamiprid and Its Metabolites in Crops by Gas Chromatography

Abstract

An analytical method has been developed for the determination of acetamiprid, (E)-N¹-[(6-chloro-3-pyridyl)methyl]-N²-cyano-N¹-methylacetamidine (ATP) and its metabolites, IM-2-1, IM-0, IC-0 and IM-0-Glucose in crops by gas chromatography (GC). ATP and its metabolites in crops were extracted with methanol and derivatized to methyl 6-chloronicotinate (IC-0-Me) through alkali hydrolysis, potassium permanganate oxidation and then esterification by diazomethane, followed by column chromatography clean-up and GC determination. The limit of detection was 0.01ppm and the recoveries of fortified samples ranged from 74 to 92%.