Effects of N-Demethylation of Pamamycins on Aerial Mycelium-Inducing and Growth Inhibition Activities

Abstract

Using a combination of ODS and NH₂ HPLCs, we isolated new pamamycin derivatives from the culture broth of Streptomyces alboniger IFO 12738. MS spectroscopy showed that their structures were de-N-methylpamamycins with the aid of labeled compounds prepared in a methionine-methyl-d₃ feeding culture of S. alboniger. De-N-methylpamamycins had stronger aerial mycelium-inducing activity and weaker growth-inhibition activity than the corresponding pamamycin with the same alkyl group arrangement.