In Vitro Formation of S-Alkyl Phosphorodithiolate and Dialkoxyphosphinyl Disulfide Compounds from a Dialkyl Phosphorodithioate by Rat Liver Microsome-NADPH System

Abstract

In vitro metabolism of S-(4-chlorophenyl) diethyl phosphorodithioate by rat liver microsomal mixed-function oxidase system gave the metabolites, S-(4-chlorophenyl) S-ethyl ethyl phosphorodithiolate and 4-chlorophenyl diethoxyphosphinyl disulfide, in addition to the oxon. An analytical procedure was developed for analyzing these metabolites which included the derivatization of the unstable phosphinyl disulfide with diazomethane and UV irradiation. Such S-alkyl isomer and phosphinyl disulfide compounds must be considered as the potential bioactive metabolites of thiophosphorus pesticides.