Visualization of metabolite change in skeletal muscle by contraction using imaging mass spectrometry

抄録

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a mass spectrometry-based imaging technique used to visualize the distribution of biomolecules without the need for extraction, purification, separation and labeling of the molecules. This technique can reveal the distribution of hundreds of ion signals in a single measurement, and also helps in understanding metabolite structure, overcoming the inadequate imaging probes for small metabolites. The versatility of MALDI-IMS has opened up new frontiers in several fields, especially in lipidomics. Lipids in skeletal muscle play a fundamental role in both normal muscle metabolism and disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. In this review, we show the applications of MALDI-IMS to mouse skeletal muscle for detecting changes in lipids induced by contraction.