Methyl-β-cyclodextrin Prevents Ca2+-Induced Ca2+ Release in Smooth Muscle Cells of Mouse Urinary Bladder

Shingo Hotta; Hisao Yamamura; Susumu Ohya; Yuji Imaizumi

doi:10.1254/jphs.SC0060213

Short Communications

Methyl-β-cyclodextrin Prevents Ca²⁺-Induced Ca²⁺ Release in Smooth Muscle Cells of Mouse Urinary Bladder

Shingo Hotta, Hisao Yamamura, Susumu Ohya, Yuji Imaizumi

著者情報

キーワード: methyl-β-cyclodextrin, Ca²⁺-induced Ca²⁺ release, smooth muscle

ジャーナルフリー

2007 年 103 巻 1 号 p. 121-126

DOI https://doi.org/10.1254/jphs.SC0060213

View "Advance Publication" version (January 1, 2007).

詳細

抄録

We examined the effects of methyl-β-cyclodextrin (MβCD) on Ca²⁺-induced Ca²⁺ release (CICR) in smooth muscle cells (SMCs) of mouse urinary bladder (UB). Short depolarization of UBSMCs under voltage-clamp elicited several local Ca²⁺ transients (Ca²⁺ hot spots) via CICR within 20 ms in discrete sub-sarcolemmal areas. Then, the Ca²⁺ wave spread to whole areas. The pretreatment with 10 mM MβCD significantly attenuated Ca²⁺ hot spots in UBSMCs and reduced contraction by single direct electrical pulse stimulation in UBSM strips. MβCD may prevent CICR by attenuating the coupling between voltage-dependent Ca²⁺ channels and ryanodine receptors in Ca²⁺ hot spot areas.

責任著者(Corresponding author)

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