THE UPTAKE OF CATECHOLAMINES BY SUBCELLULAR GRANULES IN THE BRAIN STEM

Abstract

1. The uptake of ¹⁴C-labelled dopamine and norepinephrine by the subcellular granules in brain stem was investigated.
2. The uptake was observed when the granules were incubated at 30°C and not occurred at 0°C. Much more dopamine was taken up than norepinephrine by the granules.
3. Reserpine inhibited the uptake of both dopamine and norepinephrine, but oxypertine (Win 18501-2) inhibited only the uptake of norepinephrine at a very low concentration. Iproniazid and adenosine triphosphate influenced the uptake of norepinephrine to some extent.
4. Preincubation of the granules with iproniazid rather potentiated the inhibitory action of reserpine on the uptake of norepinephrine.