Abstract
In liver microsomes of normal rats, both NADH-ferricyanide dehydrogenase and NADH-cytochrome c reductase were almost completely solubilized by addition of 0.5% DOC. Solubilization patterns were little affected by CCl4 injection. In membranes, solubilization of dehydrogenase and reductase was less than that in microsomes. CCl4 administration lowered the solubility of the dehydrogenase in membranes and increased three-fold the ratio of the reductase to the dehydrogenase remaining in the non-solubilized fraction. The reductase in membranes from CCl4 treated rat liver was found to be more resistant to solubilization by DOC. The possibility of accumulation of NADH-cytochrome c reductase in plasma membranes after CCl4 administration was discussed.
