HISTAMINE AND 5-HYDROXYTRYPTAMINE RELEASE IN ANAPHYLAXIS OF GUINEA-PIG TISSUES

Abstract

Histamine and 5-HT releases in in vitro anaphylaxis were measured spectrophotofluorometrically by immersing passively and actively sensitized guinea-pig small intestine, lung and aorta in Tyrode solution containing antigen. Histamine was released from sensitized tissues by an antigen. Values from three tissues were almost the same in both passive and active sensitizations. In passive sensitization, histamine and 5-HT were released spontaneously from tissues during and after sensitization. In active sensitization, histamine release from tissues increased along with an increase in concentration of antigen, while no increase was seen with 5-HT. 5HT was released only spontaneously from both passively and actively sensitized tissues.