DIVALENT CATION TRANSPORT IN KIDNEY SLICES. I. PROPERTIES OF CALCIUM TRANSPORT IN SLICES OF RAT KIDNEY CORTEX AND THE EFFECTS OF DIURETICS

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Correlative studies on transports of Ca²⁺ and Na⁺ and the properties of Ca²⁺ transport were carried out in rat kidney cortex slices. Ouabain had no effect on the Ca²⁺ transport but did inhibit the Na⁺ transport extensively. With addition of 2, 4-dinitrophenol to the incubation medium, or under the anaerobic conditions, the effluxes of Ca²⁺ and Na⁺ were inhibited while the Ca²⁺ influx was enhanced significantly. Sulfhydryl inhibitors such as ethacrynic acid, mersalyl and p-chloromercuribenzoic acid suppressed Ca²⁺ efflux and stimulated Ca²⁺ influx. When the slices of kidney cortex were treated with these inhibitors, there was a reduction in the content of cellular ATP. The present results suggest that Ca²⁺ transport may be partly independent of Na⁺ transport, and that Ca²⁺ efflux may require energy-yielding processes.