Autoradiographic Evidence for the Interaction of SM-3997 with 5-HT_1A Receptors in the Rat Brain

Abstract

[³H]8-OH-DPAT binding to rat brain sections and inhibition by SM-3997 were investigated. Very high densities of [³H]8-OH-DPAT binding sites were found in the dentate gyrus, entorhinal cortex, dorsal raphe, interpeduncular nucleus and lateral septum. In contrast, their densities were sparse in the substantia nigra, caudate putamen and choroid plexus. In the presence of 1 μM of SM-3997, [³H]8-OH-DPAT binding was strongly inhibited in all the brain structures we examined. These results indicate that SM-3997 binds to 5-HT_1A receptors of rat brain sections.