Effect of Ebselen on Bovine and Rat Nitric Oxide Synthase Activity Is Modified by Thiols

Ryuichi Hattori; Yoshiki Yui; Eiji Shinoda; Reiko Inoue; Takeshi Aoyama; Hiroyuki Masayasu; Chuichi Kawai; Shigetake Sasayama

doi:10.1254/jjp.72.191

Ryuichi Hattori, Yoshiki Yui, Eiji Shinoda, Reiko Inoue, Takeshi Aoyama, Hiroyuki Masayasu, Chuichi Kawai, Shigetake Sasayama

著者情報

キーワード: Nitric oxide synthase, Ebselen, Thiol

ジャーナルフリー

1996 年 72 巻 2 号 p. 191-193

DOI https://doi.org/10.1254/jjp.72.191

詳細

抄録

The inhibition of nitric oxide synthase (NOS) by ebselen, 2-phenyl-1, 2-benzisoselenazole-3(2H)-one, was reversed by the addition of 10 M dithiothreitol, suggesting that ebselen reacts with a critical thiol group of NOS in the inhibitory mechanism. In the presence of 10^minus;4 to 10^minus;3 M dithiothreitol, ebselen dose-dependently enhanced NOS activity, implicating another interaction of ebselen with NOS under these conditions. Thus, the effect of ebselen on the NOS activity is modified by thiols.

責任著者(Corresponding author)

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