1. The vacuole appears according to the condition in which the yeast-cell exists.
2. In the vacuole there are nucleolus-like body, toluidine-blue stained bodies and neutral red stained bodies. They are recognized when adequate staining method is used.
3. The nucleus is attached to one side of the vacuole and contains centrochromatin which changes into chromosomes in mitosis.
4. At the prophase of mitosis the centrochromatin changes into two chromatinic bodies. Sometimes the nucleus shows spireme in its interior. At metaphase the nucleus changes into four chromosomes which sometimes conjugate two by two.
5. At the metaphase the chromosomes appear in the spindle which is situated on the surface of the vacuole. So the chromosomes overlap on the intravacuolar bodies.