ウサギ膜性腎症および馬杉腎炎におけるin vitro guinea pig complement fixationに関する研究

抄録

The purpose of this article is to evalute the biological nature of the immune complexes, in glomeruli of experimental glomerulonephritis such as rabbit membranous nephropathy, induced by daily injection of BSA, and the rabbit Masugi nephritis produced by duck or guinea pig nephrotoxin, using in vitro guinea pig complement fixation test. The obtained results were compared with the conventional immu-nofluorescent method, light and electron microscopical observations.The result are as follows ;1. In the cases of the rabbit membranous nephropathy, the in vitro activation of the guinea pig complement was noted during of BSA administration and even in 35 weeks after suspension of BSA injection, along with the glomerular deposition of the rabbit IgG and complements.2. The in vitro guinea pig complement fixation test on the glomeruli of the Masugi nephritis revealed also positive findings especially in autologous phase but the cor-relation to the deposition of the rabbit IgG and C3 was not noted, especially in the animals received duck nephrotoxin.3. The main activating pathway of the guineatpig complement system on the glomeruli was concluded to be the classical one, in the rabbit membranous nephropathy. On the other hand, not only the classical pathway but also the alternative one was noted at the early stage of the autologous phase in the Masugi nephritis, but at the late stage of it, the alternative pathway was no more noted in glomeruli.4. The in vitro guinea pig complement fixation test was seemed to be the one of the valuable method to evaluate the pathologic activity of the immune complex in the rabbit membranous nephropathy and the rabbit Masugi nephritis.