Urinary glycoproteins in renal diseases

Abstract

Urinary glycoproteins of patients with renal diseases were analysed by means of SDS-polyacrylamide microgel electrophoresis method, and it was found that excretory patterns of urinary glycoproteins had, to some extent, a relationship with pathological changes in kidney tissue obtained by biopsy. It was also observed that seven glyco proteins: GP-1 (IgG, M.W. 160, 000), GP-2 (transferrin, M. W. 90, 000), GP-3 (M. W. 73, 000), GP-4 (M. W. 60, 000), GP-5 (M. W. 49, 000), GP-6 (α1-microgloburin, M. W. 33, 000) and GP-7 (M. W. 31, 000) were separated in urine. In healthy control, only GP-2 was observed faintly. In minimal change, GP-4 was peaked, and GP-5 was predominant in focal glomerular sclerosis and IgA nephropathy. In membrano-proliferative glomerulonephritis, GP-4 was high, whereas GP-6 was elevated in membranous nephropathy. These data indicates that there is a tendency of elevated GP-5 in mesangial change and raised GP-6 in glomerular membrane change.