Systemic Hemodynamics in glycerol-induced acute renal failure in the rats

Abstract

The time course measurement of blood volume (BV), cardiac output (CO) and regional blood flow (BF) was carried out on the glycerol-induced acute renal failure (ARF) rat, which is regarded as one of the experimental ischemic ARF models. Comparison was made between the above mentioned group and that on long term saline loading to study the pathologic physiology of ARF from the standpoint of hemodynamics. Male Wistar rats were divided into those on tap water (water group) and those on saline (saline group), both of which were given intramuscular injection of 50%glycerol (GL) at the ratio of 1 ml/100 g of body weight. BV was measured by ¹²⁵1-RISA, and CO and BF by microsphere method. BV was significantly decreased in both groups 4 hours after GL injection and did not recover even after 48 hours. CO was decreased by 40% in both groups after 4 hours but began to recover at 10 th hour to show a significant increase over the control of er 48 hours. BF to the kidney, liver, skeletal muscle and extremity showed different pattern between the 2 groups. Renal blood flow (RBF) in water group was decreased by half after 4 hours and showed the lowest value after 10 hours. It recovered to only 80% of the control after 48 hours. RBF in saline group showed the lowest value after 4 hours but began to recover after 10 hours. It showed a significant increase over the control as well as the water group at the same period. Hepatic arterial flow (HAF) in wer group was decreased to 50% of the control after 4 hours whereas in saline group it always showed higher values than those of the control. BF to the skeletal muscle and extremity in water group maintained the control values up to 10 hours afterwards while it was decreased in saline group to half of the control at 4 th and 10 th hours. Long term saline loading was quite effective in restraining the progress of glycerol induced ARF. From the standpoint of hemodynamics, it is suggested that the recovery of RBF in the early stage, the increase in HAF and the decrease in BF to the skeletal muscle and extremity in the early stage, all of which occurred in saline group only, are related to the inhibitory mechanism against ARF.