1987 年 29 巻 3 号 p. 261-270
The immunofluorescence method is indispensable for the diagnosis of IgA nephropathy, but this method has several weaknesses. In recent years, therefore, the peroxidase-antiperoxidase method, avidin-biotin peroxidase complex method, and other enzyme-labeled antibody methods have attracted much attention as procedures which might compensate for these weaknesses . The authors therefore studied the pattern of deposition of IgA, IgA subclass and complement in 34 cases of IgA nephropathy by the avidin-biotin-peroxidase complex method using paraffin-embedded tissues . The antisera employed were rabbit anti-human IgA (Behring), sheep anti-human IgA1, IgA2 (Nordic), rabbit anti-human C1q, properdin and C9 (Behring) . The following results were obtained.1) IgA deposits were observed in all cases (100%) . In 79.4% of the cases, lgA deposits were found only in the mesangial area, while in 20.6% of the cases they were also noted in the capillary wall .2) The intensity of IgA deposition revealed a high correspondence with the immunofluorescence method.3) The positive rate of IgA1 and IgA2 was 97.1% and 44.1%, respectively, and their sites of deposition corresponded with those of IgA . They showed a significant positive correlation with the intensity of IgA deposition.4) The positive rate of C1q, properdin, and C9 was 58.8%, SS.2% and 91.2% respectively, and their sites of deposition corresponded with those of IgA . The above findings suggest that the avidin-biotin-peroxidase complex method can be adequately applied to the diagnosis of IgA nephropathy.
