Effects of different fatty acids treatment on fat accumulation and adipogenesis of sheep preadipocytes

Abstract

The effects of different fatty acid treatments on fat accumulation and adipogenesis of sheep preadipocytes were investigated.  Fat accumulation in the sheep preadipocytes was increased by differentiation induction medium treatment containing palmitic acid (100 µM).  Amount of fat accumulation in sheep preadipocytes was not match the gene expressions of PPARγ and C/EBPα.  Gene expression pattern of aP2 was matched the amount of fat accumulation in sheep preadipocytes.  Furthermore, fatty acids treatments modulated the gene expression of some inflammation related factors.  These results suggest that fatty acids are not directly involved the lipid accumulation, but regulate the inflammatory factors secretion in sheep preadipocytes.