1956 Volume 47 Issue 2 Pages 102-110
Such substances as the emulsion of the human tuberculous kidney and of normal one as. the control, BCG, sterilized tubercle bacilli, and tuberculin were injected into normal adult male rabbits. The changes caused thereby in phosphatase reactions of neutrophilic leucocytes inn peripheral blood smears were observed by the method of Takeuchi et al, at pH 5.6 and 9.3. The phosphatase activities were also measured by Bodansky modified method (pH 5.0 and pH 9.3).
1) In normal adult male rabbits, both acid and alkaline phosphatase reactions appeared mainly in plotoplasm of neutrophilic leucocytes. The intensity of phosphatase reaction varied considerably according to individuals. An appearance of moderately and strongly positive reaction was common.
2) In the cases of rabbits injected with normal kidney emulsion, a change in the phosphatase reactions of neutrophilic leucocytes was, if any, absent in general when injected with tuberculous kidney emulsion, however, the phosphatase reactions of leucocytes showed a considerable increase.
3) An injection with BCG, sterilized tuberclous bacilli, tuberculin caused only a slight change in phosphatases.
4) An injection with tuberculous or non-tuberculous kidney emulsion, BCG and tuberculin caused only a slight change in the activity of phosphatase.
5) The increase in phosphatase reaction in leucocytes by tuberculosis of kidney and other organs may have a close relation with tuberculous inflammation. The phosphatase may have a cleaning action of tuderclous lesions.
