COLORIMETRICAL STUDIES ON METACHROMATIC REACTION OF URINE

III. PRE-AND POST-OPERATIVE OBSERVATION ON URINE OF RENAL TUBERCULOSIS

Abstract

Metachromatic reaction of urine in pre-and post-operative stages was investigated on 8 cases of renal tuberculosis, using the carbolfuchsin and carbolnileblue solution. The change of the color was observed macroscopically and colorimetrically. Comparing the differences of color between pre-and post-operative stages, the followings were obtained.
1) Carbolfuchsin: In pre-operative stadium, 7 cases out of 8 were positive and one was suspicious. In post-operative stadium, 1 case was indistinct and other 7 cases were negative. On CIE chromaticity diagram almost all cases except one gradually shifted from preoperative site to normal site. The color difference between pre-and post-operative stages generally became greater with the time of course.
2) Carbolnileblue: In pre-operative stage, 7 cases out of 8 were positive and one was suspicious. In post-operative stage, 3 cases were negative, 2 cases were suspicious and 3 were positive. However, on CIE chromaticity diagram, 7 cases except one shifted from pre-operative site to normal site. The color difference between pre-and post-operative stages gradually got greater with the time of course.
From the above mentioned, it is considered that the results of macroscopic judgement on metachromatic reaction of urine were well in accordance with that of colorimetry, and the deviation gradually approached to normal site after the operation.
It is confirmed that metachromatic reaction will be able to apply for the diagnosis and prognosis of renal tuberculosis, and furthermore, the carbolfuchsin solution is definitely superior to the carbolnileblue.