From the experiences of the metachromatic reaction of urine, the authors recognized that there were slight differences in judgement of the alteration of color-tone depending upon the examiners. The authors used the colorimetric method as means of color indication in order to get an accurate judgement of the reaction making up for the foregoing defect.
The metachromatic reaction was performed in 29 cases as follows: 8 cases of normal persons, 3 cases of non-tuberculous urogenital diseases, 5 cases of tuberculous urogenital diseases, before operation, and 13 cases 1-2 years after nephrectomy for renal tuberculosis. Moreover, the authors measured colorimetorically the color-tone obtained from these materials, then plotted these results on CIE chromaticity diagram.
As the results of this examination, a close agreement was recognized between two judgements by the colorimetric method and the macroscopic method investigated by Nagata and Yamamoto.

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As mentioned in the first report, as Desbordes had observed the alteration of the color of urine with the use of nileblue solution, the authors also applied carbolnileblue solution instead of carbolfuchsin solution for the original method of metachromatic reaction of urine. The alteration of color-tone was observed both macroscopically and colorimetrically. After the results obtained by the colorimetrical method was indicated on CIE chromaticity diagram, the possibility to apply carbolnileblue solution to this reaction was studied as well as in the carbolfuchsin solution.
That is, the metachromatic reaction of urine was performed using Nagata and Yamamoto's original method with the use of carbolnileblue solution in 43 cases as follows: 9 cases of normal persons, 7 cases of dermatopathy, 9 cases of nontuberculous urogenital diseases, 10 cases of tuberculous urogenital diseases before operation, and 8 cases less than 1 year after nephrectomy for renal tuberculosis. In this judgement, the cases revealing turbid with blue greenish tinge was considered positive, the cases in transparent deep blue, negative, and the intermediate cases, suspicious.
The following results were obtained: although the peculiar positive reaction was observed in tuberculosis of kidney with the use of carbolnile blue solution, when comparing this reaction with that of carbolfuchsin solution, it seems to be difficult to obtain macroscopically an accurate judgement.
However, in this examination with the use of carbolnileblue solution, both results obtained by the colorimetrical method and the macroscopic method were almost agreeable.

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Metachromatic reaction of urine in pre-and post-operative stages was investigated on 8 cases of renal tuberculosis, using the carbolfuchsin and carbolnileblue solution. The change of the color was observed macroscopically and colorimetrically. Comparing the differences of color between pre-and post-operative stages, the followings were obtained.
1) Carbolfuchsin: In pre-operative stadium, 7 cases out of 8 were positive and one was suspicious. In post-operative stadium, 1 case was indistinct and other 7 cases were negative. On CIE chromaticity diagram almost all cases except one gradually shifted from preoperative site to normal site. The color difference between pre-and post-operative stages generally became greater with the time of course.
2) Carbolnileblue: In pre-operative stage, 7 cases out of 8 were positive and one was suspicious. In post-operative stage, 3 cases were negative, 2 cases were suspicious and 3 were positive. However, on CIE chromaticity diagram, 7 cases except one shifted from pre-operative site to normal site. The color difference between pre-and post-operative stages gradually got greater with the time of course.
From the above mentioned, it is considered that the results of macroscopic judgement on metachromatic reaction of urine were well in accordance with that of colorimetry, and the deviation gradually approached to normal site after the operation.
It is confirmed that metachromatic reaction will be able to apply for the diagnosis and prognosis of renal tuberculosis, and furthermore, the carbolfuchsin solution is definitely superior to the carbolnileblue.

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Hematuria is an important clinical sign encountered not only in urological practice but also in almost all other clinical fields, and yet practically no attempts have been made for estimating the degree of hematuria based on precise criteria for its expression.
Consequently, the statements found in medical literature as to the severity of hematuria are extremely in adequate and the limits of danger as to life as indicated by the extent of hematuria have not been clearly defined. This is because of numerous difficulties involved in this problem in spite of its apparent simplicity.
For the purpose of expressing hematuria on a more quantitative basis, the author has established certain standards for judging the digree of hematuria in terms of heme concentration. This was done by first converting the blood present in various hematuric specimens into HbO₂ or HiCN and the data so obtained wee plotted in the form of a nomogram. By this means the amount of blood passed in the urine was roughly estimated.
This principle has furthermore, been applied to the construction of a simple colorimeter, in which the color developed in the urine specimen was compared with the colored glass filter corresponding to the known heme concentration. The details of the steps involved in this method are as follows:
1) The blood present in a hematuric sample is first converted into HbO₂ or HiCN and from the values of absorption maxima actually determined by spectrophotometry a nomogram is constructed, which enables one to find the blood concentration in terms of heme. A series of standards for estimating the extent of hematuria was set up on the basis of heme concentration. By ascertaining the tinctorial characteristics of the HbO₂ and HiCN solutions, the standard solutions and the colored glass filters for use in the contruction of colorimeter have been precisely determined.
2) In the quantitative determination of hemoglobin derivatives, the increase in the content of MetHb due to the therapeutic use of phenacetine or sulfonamides has attracted Wuch attention. In cases of hematuric due to the bleeding from the upper urinary tract or after retention in the bladder for a definite period of time, the degree of methemogiobinuria must necessarily differ from the condition obtaining in the blood stream. With reference to artificial hematuria, some experiments have been performed in vitro as well as by means of rabbit bladder. The results indicated that the amount of methemoglobin formation increases with the lapse of time but it is generally slow during the first 4 hours. Thus, although the Hb derivatives can be estimated by the HbO₂ method, the most accurate can be obtained by means of the HiCN method from the samples which remained in the bladder for a period longer than 4 hours.
3) Tinctorial characteristics of standard solutoins. The tinctorial characteristics of HhO₂ and HiCN solutions prepared from horse hemoglobin in 10 grades of heme concentration ranging from 0.1×10^-4M up to 1×10^-4M. The results indicated that the limits of principle wavelength were in the rage of 577m-597mμ for HbO₂, and of 575mμ-598mμ for HiCN solution.
Thus the range lies in a rather narrow zone from the yellow to the red areas, but the luminosity varied remarkably from 84.5% to 22% for HbO₂ and from 84.5% to 26.4% for HiCN solution.
As to the excitation purity also great variations were noted in ranges of 14%-71.6% for HhO₂ solution and of 21.8%-79.3% for Hicn solution. The spectral transmissin curves reveal the presence of two clear absorption bands at 541mμ and 576mμ for HbO₂ solution, but only one at 540mμ for HiCN solution. In the relative visibility curves, HbO₂ solution has two absorptionn bands. All these findings indicate a relative labilty of the HbO₂ solution for colorimetry. In

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The purpose of this study is to observe the influence of various tuberculous materials on serum calcium and potassium. Materials used in this study were homogenate of normal human kidney, that of tuberculous human kidney and emulsion of Gaseous substance in tuberculous kidney. Supernatant fluid of each material was subcutaneously injected into normal rabbits for 20 days every other day. Other group of rabbits, previously sensitized with BCG, was injected with tubercle bacilli into the left renal parenchyma after ascertaining the positive Römer's reaction. Serum calcium and potassium were determined in every group. The results were as follows;
1) In the group treated with normal kidney homogenate, neither the increase nor the decrease of Ca, K and K: Ca ratio was beyond the normal limits.
2) In the group treated with tuberculous kidney homogenate, remarkable decrease in Ca and temporal increase in K as well as in K: Ca ratio were observed.
3) In the group treated with tuberculous kidney homogenate, from which protein had previously been removed by heating at 100°C for 2 hours, a temporal decrease in Ca and a temporal increase in K as well as in K: Ca ratio were observed. These changes were, however, lower that in the group treated with unheated homogenate.
4) Specific precipitin was proved in serum of rabbits treated with tuberculous kidney homogenate. However, the heated homogenate did not show such an antigenicity.
5) In the group treated with tuberculous kidney homogenate, considerable decrease in Ca and increase in K and K: Ca ratio were brought about by an intravenous injection of a small amount of the same homogenate. On the other hand, considerable increase in Ca and decrease in K and K: Ca ratio were brought about by an intravenous injection of a small amount of heated homogenate in the majority of rabbits which had been sensitized with the same antigen.
6) In the group treated with Gaseous substance, decrease in Ca, increase in K and temporal increase of K: Ca ratio were observed.
7) In the group injected with tubercle bacilli in renal parenchma after a BCG sensitization, serum Ca increased considerably 20 days after the injection of tubercle bacilli. There-after, however, serum Ca was apt to decrease gradually. The serum K and K: Ca ratio in this group increased immediately after the injection either of BCG or tubercle bacilli. They decreased, however, 20 days after the injection in case of tubercle bacilli. They increased again thereafter. In case of BCG injection, a high level of K and K: Ca ratio was maintained without any temporal decrease.

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