EXPERIMENTAL STUDIES ON THE METACHROMATIC REACTION OF URINE
I. STUDIES ON THE METACHROMATIC REACTION OF THE URINE OF EXPERIMENTAL TUBERCULOSIS RABBITS
1958 Volume 49 Issue 6 Pages 540-553
Details
1958 Volume 49 Issue 6 Pages 540-553
The author and his collaborators have reported that tuberculosis patients show a high percentage of Nagata and Yamamoto's metachromatic reaction of urine and the reaction of the renal tuberculosis patients becomes gradually negative following the elapse of time after nephlectomy. Colorimetric investigation was also made on the changes produced in the tone of the coloring of the reaction.
In the present paper, macroscopical and colorimetric observations were made on the metachromatic reactions of the urine collected prior to and after the inoculation of tubercle bacilli, a human strain H37RV and a bovine strain Miwa, into the auricular vein of 7 normal rabbits and into the left renal artery of 5 normal rabbits. As control, 1 to 3cc of physiological saline was inoculated into either the auricular vein or the renal artery of 4 normal rabbits. As staining solution, carbol fuchsin or carbol nilblue was used. Stated in the following are the results obtained thereof:
1. When carbol fuchsin was used as staining solution:
All the 7 rabbits received the inoculation of tubercle bacilli into the auricular vein presented positive metachromatic reaction by the end of the 4th week after inoculation. While, all the 5 rabbits received the inoculation into the renal artery showed positive reaction before the end of the 3rd week. Colorimetric findings confirmed the results of macroscopic observations.
2. When carbol nilblue was used as staining solution:
Metachromatic reaction of all the rabbits received the inoculation of tubercle bacilli either into the auricular vein or into the renal artery became positive within 3 weeks after inoculation.
3. While, the reaction of all the control rabbits received the inoculation of physiologicalsaline either into the auricular vein or into the renal artery remained negative.
4. When carbol nilblue was used as a staining solution of the metachromatic reaction of urine, a distinct difference was produced between the positive and negative reactions immediately after starting the test, but the tone of the coloring of reaction easily changed following the elapse of time.
On the other hand, little changes were produced in the tone of the reaction when carbol fuchsin was used as the staining solution.
