EXPERIMENTAL STUDIES OF THE EFFECTS OF ANTIHISTAMINICS ON URINARY ECHERICHIA COLI

Abstract

The treatment of infection in the field of urology has made outstanding progress due to the successive introduction of various antibiotics and chemotherapeutic agents in therapy in recent years; these agents have had dramatically curative effects. On the other hand, however it seems that problems remain unanswered as to the appearance of resistant strains, the occurrence of superinfection and the after-effects of the drugs.
In 1959 Yamamoto et al. carried out studies on intestinal gas, which prevents roentgen diagnosis from being made, and found that there is an abnormally great increase in the amount of intracolonic gas following the administration of antihistaminics. They regarded intestinal flora and especially Echerichia coli as being responsible for that increase. Echerichia coli which was isolated from the patients were observed to produce gas in an abnormally great amount on glucose broth containing 10γ of antihistaminics per cc, as compared with the control. They pointed out that the daily dose of antihistaminics activate intestinal Echerichia coli. On treatment of urinary coli infection with antihistaminics the present author observed exacerbation of the symptoms of the vesicle in 2 of the 5 cases. The medical literature home and abroad reveals that Echerichia coli is most responsible for infection in the field of urology. When urinary coli infection occurs, it appears that the symptoms of the vesicle may exacerbate if antihistaminics are injected because of other reasons. Using 10 strains of Echerichia coli which were isolated from cases of urinary coli infection and the Ikeda strain of Echerichia coli (control) which was supplied by the Department of Bacteriology in our university, the present author observed the effects of antihistaminics upon these strains by means of in vitro experiments and animal vaccination. This paper presents a description of the results. Results:
1. No changes worth mentioning were produced on the imvic system and other properties by 10 strains of urinary Echerichia coli and the Ikeda strain of Echerichia coli which were cultivated on glucose broth containing antihistaminics.
2. The sensibility test by the use of 10 antibiotics, including penicillin, revealed as follows: Of 110 strains of Echerichia coli which were cultivated on media containing restamine, 45 showed their resistance against these antibiotics; 23 exhibited sensibility; and 42 were constant. Of 110 strains of Echerichia coli which were cultivated on media containing allergin, 29 were resistant; 13, sensible; and 68, constant. The number of the former strains which exhibited their resistance against the antibiotics was greater than that of the latter.
3. When the lethal test was performed on mice, it was found that deaths from strains of Echerichia coli which are cultivated on media containing antihistaminics show a greater increase than in the case of the original strain.
4. The necrosis test which was performed on rabbits disclosed that strains of Echerichia coli which are cultivated on media containing antihistaminics cause the formation of necrosis in 2 of the 3 rabbits more remarkably than the original strain does.
5. The inspection of the growth curve of urinary Echerichia coli indicated that strains of Echerichia coli which are cultivated on media containing antihistaminics show a more significant increase in the number of living bacteria 6 hours after cultivation than the original strain does.
6. Accordingly, much precaution should be taken against these matters when urinary coli infection is treated with antihistaminics.