1967 Volume 58 Issue 10 Pages 1003-1009
The effects on tumor of chromomycin A3 (Cm A3), mytomycin C (M. M. C) and nitromin (N. M) were studied. For this rats were employed the urinary bladders of which were transplanted with Yoshida sarcoma and AH 130 hepatoma. The following results were obtained:-
1. The relationship between the effect of the drug and its administration time was checked by comparing the 3 groups, the first beginning to receive the drug intravenously or into the peritoneum 3 prior to the transplantation, the second after the transplantation and the third 3 days after the transplantation.
The best results were obtained among the group which began to receive the drug immediately after the transplantation.
2. In rats which were transplanted with AH 130, 15γ, 30γ and 50γ/kg/day of Cm A3 was administered for 5 days immediately after the transplantation.
In the case of administration into the peritoneum the effect of life prolongation was rarely seen, while in the case of intravenous administration the effect was good with 15γ and 50γ/kg administration.
3. In rats which were transplanted with AH 130, the effect of M. M. C was observed by giving 0.1mg, 0.2mg, 0.5mg, 1mg and 2mg/kg of M. M. C into the peritoneum, and intravenously 0.1mg and 0.5mg/kg/day for 5 days continuously immediately after the transplantation. A good response was seen in groups which received 0.1mg, 0.2mg and 0.5mg of M. M. C into the peritoneum and in groups which received the drug intravenously.
4. The effect of N. M also was checked in the same way as M. M. C. In the case of N. M administration with the dose of 1mg, 3mg and 5mg, the effect of life prolongation was remarkable both in intravenous groups and intraperitoneal groups.
5. The effect of life prolongation was checked in the case of combined use of two drugs; 15γ/kg/ day of Cm A3, 0.1mg/kg/day of M. M. C and 1mg/kg/day of N. M were combined so that two of these three drugs constituted one combination, thus 6 combinations being formed, each combination was administered intraperitoneally or intravenously. In the case of intraperitoneal administration no prolongation of life was observed compared to single administration of each drug. However, in the case of intravenous administration good results were obtained by combined use of drugs, especially in the combined use of N. M and Cm A3, and also in the combinations of N. M and M. M. C.
In these two combinations 30 day survival was 92%.
