The effects on tumor of chromomycin A₃ (Cm A₃), mytomycin C (M. M. C) and nitromin (N. M) were studied. For this rats were employed the urinary bladders of which were transplanted with Yoshida sarcoma and AH 130 hepatoma. The following results were obtained:-
1. The relationship between the effect of the drug and its administration time was checked by comparing the 3 groups, the first beginning to receive the drug intravenously or into the peritoneum 3 prior to the transplantation, the second after the transplantation and the third 3 days after the transplantation.
The best results were obtained among the group which began to receive the drug immediately after the transplantation.
2. In rats which were transplanted with AH 130, 15γ, 30γ and 50γ/kg/day of Cm A₃ was administered for 5 days immediately after the transplantation.
In the case of administration into the peritoneum the effect of life prolongation was rarely seen, while in the case of intravenous administration the effect was good with 15γ and 50γ/kg administration.
3. In rats which were transplanted with AH 130, the effect of M. M. C was observed by giving 0.1mg, 0.2mg, 0.5mg, 1mg and 2mg/kg of M. M. C into the peritoneum, and intravenously 0.1mg and 0.5mg/kg/day for 5 days continuously immediately after the transplantation. A good response was seen in groups which received 0.1mg, 0.2mg and 0.5mg of M. M. C into the peritoneum and in groups which received the drug intravenously.
4. The effect of N. M also was checked in the same way as M. M. C. In the case of N. M administration with the dose of 1mg, 3mg and 5mg, the effect of life prolongation was remarkable both in intravenous groups and intraperitoneal groups.
5. The effect of life prolongation was checked in the case of combined use of two drugs; 15γ/kg/ day of Cm A₃, 0.1mg/kg/day of M. M. C and 1mg/kg/day of N. M were combined so that two of these three drugs constituted one combination, thus 6 combinations being formed, each combination was administered intraperitoneally or intravenously. In the case of intraperitoneal administration no prolongation of life was observed compared to single administration of each drug. However, in the case of intravenous administration good results were obtained by combined use of drugs, especially in the combined use of N. M and Cm A₃, and also in the combinations of N. M and M. M. C.
In these two combinations 30 day survival was 92%.

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In order to obtain the morphological evidences of radiological findings, experimental lymphography was performed in rabbits of three groups (Brown-Pearce tumor group, acute inflammation and control)
1) Brown-Pearce group
Lymphography was performed in 6 to 16 days after transplantation. The typical metastatic pattern could be visualized in the tumor group.
2) Acute inflammation of lymph nodes could be caused by transplantation of staphylococcus into the testicle of rabbit.
The lymphogram visualized the typical pattern of inflammatory lymph nodes.
3) Above mentioned metastatic radiographic pattern and inflammatory pattern are each characteristic, and can be distinctly differentiated.
4) After roentogenographic examination, the iliac lymph nodes “en bloc” were removed and histopathological examination was performed by means of “wide-and step section”. And then radiographic-pathological correlation in the interpretation of lymphogram was analysed.
5) Marginal filling defects were demonstrated sometimes even in lymphogram of control group. These were due to the pressure by the iliac blood vessels.
6) Overlapping of the lymph nodes, especially in metastatic, does not visualize the distinct radiographic appearance. The pathological examination, “wide-and step section”, showed the correlation.
7) As the walls of the sinusoids are distended, sometimes ruptured and then pseudocysts are formed, there are sometimes island-like dense shadow in homogeneous reticular or granular pattern of the nodes.
8) Even if abnormal pattern or structure were seen in lymphogram, metastasis was not always demonstrated in the node.

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At present, there remain a vast number of renal parenchyma) diseases for which no curative operation is known.
Many of these renal parenchymal diseases affect the renal circulation primarily or secondarily, incurring renal vascular insufficiency.
Such vascular insufficiency is peripheral (parenchymal) rather than proximal (within the main renal arterial supply).
The author focused this investigations on the methods of early reestablishment of the adequate blood supply in these paticular situations.
In this investigation, twenty-four adult mongrel dogs weighing 10 to 20Kg. were devided into three groups;
1) ischemic kidney group (main experiment),
2) obstructed kidney group (that means complete obstruction of the renal artery),
3) normal kidney group.
The peripheral revascularization of the renal parenchyma attempted by implanting the artificially formed aneurysmal splenic artery.
On each group, effect of the implanted artery were evaluated by I. V. P., aortography, renal angiography, histologic examination, BUN and alterations of serum electrolytes and blood pressure.
Aortography and I. V. P. were inadequate to evaluate the revascularization.
Renal angiograms and histological studies demonstrated obvious vascular communication between implanted artery and renal vascular system on all groups.
Newly developed renal revascularization was the most pronounced on the ischemic group followed by the obstructed and the normal control group in decreasing order.
Renal angiogram and histological studies of ischemic and obstructed kidneys proved the blood from the implanted artery circulated into the Gomerulus first, and then to the renal vascular system.
And the india ink injected into the aorta entered the glomeruli under the physiological state, through the implanted artery.
Conclusions:
1) The best renal revascularization was obtained when the aneurysmal splenic artery, produced artificially by stripping the tunica externa, was implanted.
2) Vascular communication between the implanted artery and the renal vascular system was observed 14 days postoperatively.
3) Direction of the blood flow in the ischemic and the obstructed kidneys was as follows; Implanted artery— Revascularized artery— Afferent artery— Glomerulus— Efferent artery.
4) An increase in number of arteries implanted meant the better revascularization.
5) Currently studies are underway to implant more arteries utilizing, both the mesenteric and the splenic arteries for better renal revascularization, and clinnical application is forthcoming.

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The first case was 38-year-old housewife with the chief complaint of recurrent gross hematuria for 10 years prior to admission. Pelvic examination revealed no evidence of the bleeding or disease.
The second case, 64-year-old man, was admitted with gross hematuria and right flank pain. This patient had noted hematuria of 3 weeks' duration 4-1/2 years previously. Intravenous pyelogram showed some irregularity of the middle calyx on the right side.
The third case, 27-year-old woman, was admitted complaining of hematuria. She had eight episodes of gross hematuria for 5 years. Intravenous and retrograde pyelograms were repeated and found to be normal.
In three cases, transfemoral aortograms were taken, and they all showed a localized pooling-like shadow in the tumor area. Nephrectomy was performed in these cases, and pathological diagnosis was hemangioma of the kidney.
Eighteen cases of hemangioma of the kidney are collected in the Japanese literatures including our three cases, and some clinical and pathologic observation are given. Put, if more exhaustive examination of the kidney were made, more cases of so-called essential hematuria could be found to be due to hemangioma, and study of aortogram may prove to be of great value in the diagnosis of hemangioma of the kidney.

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A case of primary dermoid cyst of the urinary bladder in a 29 years olds was reported.
The tumor was pediculated and completely localized within the bladder.
Histologically, it was shown to be a dermoid cyst of the urinary bladder composed mainly of skin-like structure with cartilage, sweat gland, adipose gland and hair.
Left ovarium adhered to the bladder wall but did not infiltrate.
So this tumor was thought to be a primary dermoid cyst of the bladder.
The authors collected 59 cases of bladder dermoid cysts from Japanese literatures and analysed them statistically.

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In the previous report (40) a method of preparing fluorescent antibody to gonococcus and the results of tests for its specific activity were described. Fluorescent antibody labelled with two or three fluorescein molecules was shown to be most useful in view of its specificity and staining potency. Among stock strains of genus Neisseria and of several gram-positive cocci, only meningococcus strains (Group A, B, C, and D) were found strongly cross reactive with the fluorescent antibody solution, although, because of the natural antibody involved, a strain of staphylococcus (209P) was stained to some extent. Further studies carried out thereafter on strains which were isolated from uretheritis and cystitis patients, and revealed that the unfavourable staining of microorganisms indigenous to urethral membrane would not be likely to occur, so far as the present fluorescent antibody solution was concerned.
The present studies were undertaken to make a clinical appraisal of fluorescent antibody technique (FAT) for the detection of gonocucci in specimens taken from patients. Specimens from 126 patients were examined by both gram and fluorescent antibody staining. One hundred and twelve males were suspected of gonorrhea and 14 females were mostly the sextual partners of male patients. Cultural examinations were carried out on 86 out of 126 cases. Gram staining demonstrated the presence of gramnegative diplococci in 85 cases (67.5%), as compared to that of specifically by FAT stained diplococci in 79 cases (62.0%).
Cultural examinations of 86 patients detected the presence of gonococci in 46 cases (88.5%) on the first trial. FAT-positive, culture-negative six cases were treated with placebo and subjected to cultural examination again on the next day. With this procedure five more positive cases were detected, making in total 51 positive cases (98.1%). The isolates were identified as gonococci by oxidase reaction as well as by fermentation tests when necessary. Thus, FAT and cultrual method gave nearly identical results in both positive and negative cases. This fact led the author to believe that the FAT is as much accurate and sensitive as cultural examination. About one fourth of the patients were examined by both direct and delayed methods of FAT. Both results agreed quite well in all of the cases tested.
Eighty-five gram-negative diplococci found by gram staining probably included nine microorgainsms other than gonococci, as revealed by FAT and cutrural examination. The causes of discrepancies between the results obtained by gram staining, FAT and cultural examination were discussed in detail. The specificity, sensitivity, simplicity and rapidity of FAT in demonstrating gonococci in specimens indicated the usefulness of this procedure as a diagnostic tool for gonococcus infections.

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