ON THE STUDY OF THE IMPLANTATION OF ASCITES TUMOR IN URINARY BLADDER

REPORT 5. EFFECT OF ANTITUMOR AGENTS (2)

Abstract

The effects of Chromomycin A₃(CmA₃), Mitomycin C (M. M. C), and Nitromin on metastasis of AH 130 hepatoma implanted in the rat urinary bladder were studied by histologic examination.
The results obtained were as follows:
1) The rats which received on intraperitoneal administration of M. M. C 0.5mg/kg/day, were found to be free from metastasis, despite of tumor formation in the implanted site in the urinary bladder. The rats which received a combined administration of M. M. C 0.1mg/kg/day and Nitromin, 1mg/kg/day, into the abdominal cavity were also free from metastasis, except in the retroperitoneal lymph nodes.
2) By either single or combined intravenous administration of M. M. C, Nitromin, and CmA₃ hematogenous metastasis was prevented fairly well, but lymphogenous and continous metastases could not be satisfactorily prevented.
3) Intravenous administration of CmA₃ did not prevent metastasis to the retroperitoneal lymph nodes, lung, and spleen. The extensive metastasis in the spleen in the rats administrated with this drug was characteristic.
4) Generally speaking, each antitumor agent used in this study showed some preventive effects on the metastases except retroperitoneal lymph nodes. However, M. M. C showed a marked preventive effect on the metastasis in the retroperitoneal lymph nodes, when it was given intraperitoneally in a dosage of 0.5mg/kg/day.
From the above results it was considered that the antitumor agents had only a slight effect on the original, implanted tumor and on lymphogenous metastasis, and that these agents were more effective when they were combined with radical surgical operation.