Quinacrine系色素による蛍光性 Barr body (x-chromatin) と Fluorescent body (Y-chromatin) の研究
1972 年 63 巻 12 号 p. 1016-1038
詳細
1972 年 63 巻 12 号 p. 1016-1038
The author investigated the most suitable conditions in staining both fluorescent X-chromatin and Y-chromatin.
1) Quinacrine mustard (Q. M.) staining was far superior to Quinacrine dihydrochloride (Q. D.) staining on the incidence of X and Y-chromatin.
2) The time factor was found to be the most important for staining, for buccal smear as well as for blood smear.
3) It was also revealed that blood smear without fixation showed high incidence in fluorescent body than that with fixation if the samples were observed within a few days.
4) The incidence of fluorescent X and Y-chromatin was studied in normal males and females by applying the staining conditions described above. The results were as follows:
a) The average incidence of fluorescent Y-chromatin in normal males was 39. 8% in buccal mucosal cells with 0.5% Q. D. and 54.0% with 0.005% Q. M. On the other hand in blood smears, the average incidence was 46.1% with Q. D. and 67.3% with Q. M.
b) The average incidence of fluorescent X-chromatin in normal females was 12.0% in buccal mucosal cells with Q. D. and 17.3% Q. M. A low incidence was observed in normal males: 0.6% with Q. D. and 0.8% with Q. M. respectively.
5) The average incidence of double structure in buccal smear was 3.0±1.1%.
6) In the screening test by this fluorescent staining method for sex check up of 217 female participants in the international Winter Sports Festival held in Sapporo in 1972, one person was found to have sex chromosomal constitution of XY.
7) X and Y-chromatin test by this fluorescent staining method was applied to 15 patients with sex chromosomal anomaly and it was confirmed that this staining method was very useful for inferring sex chromosomal constitution except for mosaicism.
