BLOOD-CLOTTING AND FIBRINOLYTIC ENZYME SYSTEM IN BLOOD AND TISSUE IN EXPERIMENTAL URINARY BLADDER TUMORS IN RATS INDUCED BY ORAL ADMINISTRATION OF N-BUTYL-N-BUTANOL (4)-NITROSAMINE
1973 Volume 64 Issue 5 Pages 383-396
Details
1973 Volume 64 Issue 5 Pages 383-396
There are various theories concerning the relationship between malignant tumors and bloodclotting, fibrinolytic enzyme system and peptides associated with these enzymes.
1. A dose of 0.02ml/head/day of N-butyl-N-butanol (4)-nitrosamine (BBN) was administered orally to eight-week-old Wistar-Imamichi strain male rats for twelve weeks and then these animals were divided into three groups.
One group was not treated (control group), and the other groups received either low-molecularweight dextran (LMW-dextran), which shows lipoprotein-lipase activity and fibrinolytic activity (LMWdextran group), or p-amino-methyl-benzoic acid (PAMBA), which shows antifibrinolytic activity (PAMBA group), each three times intraperitoneally.
These animals were sacrificed at the ninth week after completion of BBN administration.
2. Incidence of experimentally induced bladder tumor in the control group, LMW-dextran group and PAMBA group were 76.5% (13 out of 17 rats), 60% (three of five) and 56% (five of nine) respectively.
3. When the group suffering from bladder tumor was compared with the other without tumor, the former had a tendency of increase in fibrinogen and fibrinolytic activity and of decrease in antifibrinolytic activity in plasma.
4. The tumor tissue had a tendency to have larger amount of fibrinogen, tissue thromboplastinlike substance and higher fibrinolytic activity than non-tumor tissue.
