CELL KINETICS OF THE BLADDER CARCINOMA
II. Effects of Thio-TEPA on Proliferative Kinetics of the Cultured Human Bladder Carcinoma Cells
1976 Volume 67 Issue 7 Pages 497-501
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1976 Volume 67 Issue 7 Pages 497-501
Effects of Thio-TEPA (TTPA), a potent alkylating agent for topical chemotherapy, were investigated in terms of proliferative kinetics of MGH-U1 cell line, the cultured human bladder carcinoma cells. The proliferating cells were exposed to various dose of TTPA for 2 hours.
10μg/ml of TTPA exhibited a temporarily static effect on the growth curve, while 50μg/ml and 100μg/ml of this agent were representative of subcidal and cidal effects, respectively. 3H-TdR uptakes by the cells exposed to subcidal as well as cidal dose of TTPA dramatically decreased, which was followed by a temporary increase 3 or 4 days later. 3H-TdR uptake following the static dose of TTPA showed an exponential increase which was higher than the control. The increased 3H-TdR uptakes by the cells after TTPA administrations might be indicative of DNA repair or prolongation of S phase.
The static dose of TTPA led to aprolongation of the cell cycle time by 32.4% as compared to the control, with the principal effect being seen in S phase duration. The static dose effect of this agent would be much suggestive in the light of therapeutic manipulation of the bladder carcinoma.
