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Volume 67, Issue 7
Displaying 1-9 of 9 articles from this issue
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I. Cell Cycle Analysis of the Cultured Human Bladder Carcinoma CellsTetsuro Kato1976 Volume 67 Issue 7 Pages 491-496
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSCell cycle analysis was performed on two established cell lines of human bladder transitional carcinoma, MGH-U1 and T24 cells. Since MGH-U1 cell was successfully established in the urological laboratory of the Massachusetts General Hospital in 1972 and T24 cell was originally developed in Sweden in 1970, both of them have been passaged in culture several hundred times.
The cells were cultured on round cover slips for three days, autoradiograms were prepared after pulse-labeling with 3H-TdR and fraction of labeled mitoses (FLM) were calculated every 2 hours through 36 hours. The FLM curves were analyzed by the empirical half-height level method as well as Mendelsohn-Takahashi's asymmetry method which is a global type of FLM analysis based on Takahashi generalized model. The results were as follows,
MGH-U1 cells; (TC) asym., corr.=26.2hr, (TS) area, corr.=8.2hr, (TG2+0.7M) est. level=3.6hr, (TG1+0.3M) est. level=14.4hr, (CV) est.=34.3%. By the half-height level method (TC)=18.7hr, (TS)=7.9hr, (TG2+0.5M)=3.5hr, (TG2+0.5M)=7.3hr.
T24 cells; (TC) asym., corr.=26.6hr, (TS) area, corr.=8.8hr, (TG2+0.7M) est. level=4.1hr, (TG1+0.3M) est. level=13.7hr, (CV) est.=31.9%. By the half-height level method (TC)=18.9hr, (TS)=8.8hr, (TG2+0.5M)=3.9hr, (TG2+0.5M)=6.2hr.
The data did not show any distinct differences of the cell cycle parameters between MGH-U1 and T24 cell lines.
A remarkable discrepancy was observed in (Tc) asym., corr. and (Tc) half-height of each cell line. Although there has been no assurance that the asymmetry method is biologically correct up to now, Mendelsohn and Takahashi tested this method repeatedly and mathematically proved that it had much less error than that of the traditional boundary method.View full abstractDownload PDF (1963K) -
II. Effects of Thio-TEPA on Proliferative Kinetics of the Cultured Human Bladder Carcinoma CellsTetsuro Kato1976 Volume 67 Issue 7 Pages 497-501
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSEffects of Thio-TEPA (TTPA), a potent alkylating agent for topical chemotherapy, were investigated in terms of proliferative kinetics of MGH-U1 cell line, the cultured human bladder carcinoma cells. The proliferating cells were exposed to various dose of TTPA for 2 hours.
10μg/ml of TTPA exhibited a temporarily static effect on the growth curve, while 50μg/ml and 100μg/ml of this agent were representative of subcidal and cidal effects, respectively. 3H-TdR uptakes by the cells exposed to subcidal as well as cidal dose of TTPA dramatically decreased, which was followed by a temporary increase 3 or 4 days later. 3H-TdR uptake following the static dose of TTPA showed an exponential increase which was higher than the control. The increased 3H-TdR uptakes by the cells after TTPA administrations might be indicative of DNA repair or prolongation of S phase.
The static dose of TTPA led to aprolongation of the cell cycle time by 32.4% as compared to the control, with the principal effect being seen in S phase duration. The static dose effect of this agent would be much suggestive in the light of therapeutic manipulation of the bladder carcinoma.View full abstractDownload PDF (685K) -
Toshihiro Matsumura1976 Volume 67 Issue 7 Pages 502-514
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSEffects of intrarenal arterial administration (IRA) of prostaglandin A2 (PGA2) and E2 (PGE2) on intrarenal blood flow distribution and renin release were studied in dogs anesthetized with pentobarbital. Plasma renin activity (PRA) was measured by radioimmunoassay. Intrarenal distribution of blood flow was determined by means of radioactive microsphere method.
PGA2 (0.1μg/min, IRA) caused an increase in renal blood flow (RBF) and a slight increase in urine flow (UF) without any change of renal arterial pressure (RAP). Renal venous PRA was slightly but significantly increased during PGA2 infusion into the renal artery (0.1μg/min).
Doses of PGA2 (0.5μg/min, IRA) and PGE2 (0.1μg/min, IRA) seemed to be maximum effective ones on RBF without any change of RAP. Both PGA2 (0.5μg/min, IRA) and PGE2 (0.1μg/min, IRA) increased RBF, UF and urinary sodium and potassium excretion, but none of them influenced GFR. PGA2 (0.5μg/min, IRA) increased significantly arterial and renal venous PRA. On the contrary, PGE2 (0.1μg/min, IRA) had no influence on arterial or renal venous PRA.
Concerning the intrarenal distribution of blood flow, both PGA2 (0.5μg/min, IRA) and PGE2 (0.1μg/min, IRA) resulted in an increase of flow rate in each cortex zone. But the zonal response pattern was not uniform, and it was characterized by a progressively proportional increase in flow from the superficial to the deep cortex. Thus, percent distribution was significantly changed showing redistribution of blood flow from the superficial cortex to the deep cortex.
The above results suggest that both PGA2 and PGE2 have the almost same effects on renal hemodynamics and urine formation. It is likely that PGA2 stimulates renin release but PGE2 dose not.View full abstractDownload PDF (1776K) -
Masao Osafune, Minoru Matsuda, Toshihiko Kotake1976 Volume 67 Issue 7 Pages 515-525
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSSixty cases of testicular parenchymal tumors were experienced in the Osaka University Hospital for the last 18 years (from 1957 to 1974). These cases were reviewed with regard to their clinical, histopathological and prognostic features.
1. The histopathological examination revealed that they were classified as follows; type I in 29 cases (48.5%), type II in 10 (16.7%), type III in 8 (13.3%), type IV in 7 (11.7%) and type V in 3 (5.0%) respectively, according to the pathological classification by Dixon & Moore. Of 3 non-germinal tumors out of the 60 cases, one was interstitial cell tumor and two were reticulum cell sarcomas.
2. As for the age distribution, two peaks were noted in testicular tumors, especially in cases with element of embryonal carcinoma, at the ages of 0 to 4 and 30 to 34. On the other hand, the cases with seminoma element showed one peak at the age of 30 to 34.
3. Observed 5-year survival rate in our series was 91%.
4. Although the period from the clinical onset to the definitive treatment had no apparent relationship to the clinical stage and to the prognosis, considerable relationship was observed between the clinical stage and the prognosis. Therefore, it was emphasized that the clinical staging is very important for the prognosis of testicular tumor as to determine the therapeutic procedures.
5. The observed survival rate was compared with the pathological classification and the therapeutic procedures. The result was that in non-seminomatous germinal tumor radical orchiectomy plus retroperitoneal lymphadenectomy and/or radiation therapy were for better prognosis compared with radical orchiectomy alone.View full abstractDownload PDF (1591K) -
Tsuneo Kawai, Takashi Takeda, Hideo Nakao1976 Volume 67 Issue 7 Pages 526-535
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSLactic acid dehydrogenase (LDH) activities in serum were assayed in various states of 79 cases with testicular tumors since 1967 in our hospital, and the following results were obtained.
1. Comparison of serum LDH activities before and after extirpation of testicular tumors.
i. Cases with or without metastasis of less than 8cm in diameter.
The serum LDH activities remained between 150 and 450 unit before and after extirpation in most cases with the primary tumors measuring less than 10cm in diameter. On the other hand, abnormally elevated LDH values decreased and became normal after extirpation in cases with the primary tumors measuring over 10cm in diameter (Fig. 1).
ii. Cases with metastasis of larger than 8cm in diameter.
Abnormally elevated values of LDH were still observed after extirpation of the primary lesions in all cases, which is attributed to large metastasis (Fig. 3).
2. The values of serum LDH activities were observed between 200 and 400 unit in uneventful cases after treatment (Fig. 4 & 5).
3. The values of serum LDH activities changed depending on tumor size throughout the course of cases with metastasis (Fig. 7).
4. The values of serum LDH activities were considered to reflect the clinical course in cases of the testicular tumor, but abnormally elevated values of LDH were observed only in cases with the tumor size which was larger than about 10 cm in diameter and they were useless for diagnosis in early stage.
5. No characteristic change of serum LDH activity was observed with varying histological classification, location of metastasis and method of treatment of testicular tumors.
6. The normal values of serum LDH activities of children were slightly higher than those of adults, which ranged between 300 and 600 units (Fig. 6).View full abstractDownload PDF (1131K) -
Yoshinobu Hoshino, Takehiko Yokota, Shunsuke Aoki, Kunihiro Ogura1976 Volume 67 Issue 7 Pages 536-542
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSA histological examination was carried out on the bladder neck sclerosis of the aged.
Specimens were obtained at the operation (perineal or retropublic prostatectomy with bladder neck reconstruction). Besides hematoyxlin-eosin (HE) staining, alcian blue-periodic acid Schiff (AB-PAS) staining, toluidine blue (TB) staining, van Gieson (v. G.) staining and Weigert staining were performed. The proliferation of collagen fibers, which is presumed to be a senile change, appeared to be the essential histological finding of the bladder neck sclerosis.
The proliferated collagen fibers can be observed clearly by v. G. staining even on the tissues where they are not detected by HE staining. Since the infiltration of chronic inflammatory cells is often accompanied by the proliferation of collagen fibers, it is suspected that chronic infection can induce and accelerate the sclerotic changes of the bladder neck.View full abstractDownload PDF (8706K) -
Hisashi Noutomi, Hiroshi Kanetake, Hironobu Hakariya1976 Volume 67 Issue 7 Pages 543-547
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSDuring the two months from November 1975, the outer needle of ascites trocar MIC, 648 from local market was used as a guide canal for the patients with suspected renal disease in our hospital and satisfactory results of percutaneous renal biopsy have been obtained. In this paper, the technique of the use of guide canal, the results of renal biopsy and the advantage of the use of guide canal are described and the results of this technique are compared with the results of percutaneous renal biopsy performed in Japan.
1. Renal biopsy was performed on six cases, two children and four adults, using the Tru-Cut needle (Travenol) as the biopsy needle together with a guide canal. The tissue specimen collected was 17.8mm in length on the average containing 38.8 gromeruli on the average. There was no case of accident.
2. The advantage of the use of a guide canal for biopsy is that the resistance of the kidney is felt directly on the biopsy needle by the technique corresponding to open needle biopsy because of little tissue on the way to the kidney and thus the collection of the target tissue is feasible, allowing satisfactory results even in repeated punctures. Without being influenced by the age and structure of the subject, posture at the time of biopsy and any kinds of biopsy needles, this technique is also expected to give satisfactory results when it is applied for the cases of iodine hypersensitivity in whom pyelogram is not available.
3. The use of a guide canal in this renal biopsy is believed to minimize the frequency of accident due to renal biopsies.
4. Follow-up and review of this technique are expected from those who are engaged in renal biopsy at various institutions.View full abstractDownload PDF (5307K) -
Akihiko Okuyama, Nobuo Nagai, Michiyuki Usami, Hiroaki Itatani, Shutar ...1976 Volume 67 Issue 7 Pages 548-557
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSTwo boys with Prader-Willi Syndrome were reported. Both complained of testicular retention, hypogenitalism, mental retardation, acromicria and obesity with muscular hypotonia in the newborn period. Both showed low responses of serum LH and FSH following LH-RH stimulation. Literature was reviewed briefly.View full abstractDownload PDF (4907K) -
Teruo Kadowaki, Takashi Kurita, Noboru Okuda, Hiroshi Sakaguchi, Tetsu ...1976 Volume 67 Issue 7 Pages 558-564
Published: July 20, 1976
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSThoracic kidney is extremely rare with only 95 cases reported in the literature. Recently we experienced a case of right thoracic kidney with renal calculi and various congenital anomalies, such as pulmonary sequestration, stenotic right pulmonary artery, aplastic right lower lobe, eventration of the diaphragm, and anomalous right coronary artery. Right nephrolithotomy, nephropexy, and lobectomy were performed successfully. This unusual case is described with a review of the literature.View full abstractDownload PDF (2986K)
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