蓚酸カルシウム結晶の成長を抑制する高分子物質についての研究

第2編 蓚酸カルシウム結晶成長抑制物質のラット肝よりの分離・同定

抄録

A calcium oxalate crystal growth inhibitory substance was obtained by 3M MgCl₂ extraction and ethanol precipitation. The density of this substance was found to be very high by applying the material to CsCI buoyant density gradient centrifugation.
The rat liver was homogenized in water and heated at 80°C for 30min. This active substance was applied to a DEAE-cellulose column and eluted from EADE by increasing the concentration of NaCl in the buffer. Each fraction was assayed for inhibitory activity, protein, RNA, DNA, and uronic acid.
The active fraction from DEAE was further purified by preparative disc gel electrophoresis using 3% polyacrylamide 0.5% agarose composite gel. The substance was electrophoresed faster than bromophenol blue. It became clear since this substance is very acidic.
These two characteristics along with the elution pattern from DEAF-cellulose column suggested that this material is RNA. This hypothesis was confirmed by the ultraviolet absorption spectra and the digestion experiment with RNases.