IMMUNOLOGIC ALTERATION IN BLADDER TUMORS II

PHA-INDUCED LYMPHOCYTE BLASTOID FORMATION AND PPD SKIN REACTION

Abstract

Non specific cellular immune responses in bladder cancer patients (BC), other malignant tumor patients (OMT) and benign disease patients (BD) were studied for PHA-induced blastoid formation and PPD skin reaction.
1) PHA-induced lymphocyte blastoid formation was indicated by the difference of ³H-Tdr incorporation (dpm) between PHA-stimulated tubes and non stimulated, and were studied in 16 patients with BC, 17 patients with OMT and 26 patients with BD.
The following results were obtained: The mean incorporation±SE from 7 patients with low grade BC was 2285±511.3dpm that from, 9 patients with high grade BC 1199±487.6dpm that from, OMT 781±281.6dpm and that from, BD 3712±587.3dpm. PHA-induced lymphocyte blastoid formation was reduced in high grade BC in progressed and malignant patients.
2) Delayed skin hypersensitivity reactions with purified protein derivative (PPD) were observed at 48hrs. The mean reaction with SE from 8 patients with grade I of BC was 18.0±2.44mm, that from 29 patients with grade II of BC 19.6±2.53mm that from, 19 patients with grade III of BC 15.3±3.56mm that from, 21 patients with grade IV of BC 10.5±1.70mm, and that from 47 patients with BD 19.2±1.95mm. The mean reaction from 18 patints with stage O, A of BC 16.9±3.45mm, that from 5 patients with stage B₁ of BC 16.9±7.44 that from, 12 patients with stage B₂, C of BC 9.8±1.92mm, and that from 14 patients with stage D of BC 14.4±4.01mm. PPD reaction was reduced as grade or stage of the bladder cancer progressed and in malignant patients group.
3) Some followed up cases of bladder cancer were discussed with the course although the non specific cellular immune responces of the patients with bladder cancer were generally reduced.