1980 Volume 71 Issue 4 Pages 324-329
Acid phosphatase (ACP) and its isoenzymes (Fr I, Fr II, Fr III) separated by ion-exchange column chromatography, were assayed in blood and bone marrow sera of 20 patients with prostatic cancer (PC), 3 patients with benign prostatic hypertrophy (BPH), and 6 normal males.
1) ACP activities of sera from normal males were total ACP (TAP), 0.59±0.16 Sigma unit/ml and L-tartrate labile ACP (PAP), 0.16±0.06 Sigma unit/ml. There was no significant difference of serum ACP activity among BPH, stage B and C of PC, and normal males.
Serum ACP isoenzymes in normal males were Fr I 72.4±6.5%, Fr II 14.7±7.7%, and Fr III 12.4±6.4%. The ACP isoenzyme pattern in serum of BPH was almost the same as that of normal serum. The ACP isoenzyme Fr II in serum of PC was more increased in higher stage: 20.8±3.6% in stage B, 35.5±21.0% in stage C, and 43.3±20.2% in stage D. The relationship between hormone therapy and ACP activity was discussed.
2) Bone marrow ACP (BM-ACP) activities were: TAP 1.07±0.28 Sigma unit/ml, PAP 0.17± 0.08 Sigma unit/ml in normal males, TAP 1.11±0.39 Sigma unit/ml, PAP 0.11 ± 0.06 Sigma unit/ml in BPH, TAP 16.79 ±40.30 Sigma unit/ml, and PAP 10.59± 27.89 Sigma unit/ml in PC. BM-ACP showed a higher activity than serum ACP in all cases.
BM-ACP isoenzymes in normal males were Fr I 80.2±7.0%, Fr II 12. 9±8.0%, and Fr III 8.1±6.1%. The difference between serum and bone marrow ACP isoenzyme patterns of BPH and PC was not significant.
3) The correlation between serum and bone marrow ACP activity (TAP, PAP, isoenzyme Fr II) and bone metastasis was discussed.
