HIGH-PERFORMANCE AND REVERSED-PHASE LIQUID CHROMATOGRAPHIC ASSAY FOR ADRENAL CATECHOLAMINES IN SPONTANEOUSLY HYPERTENSIVE RATS

Abstract

A highly sensitive and simplified procedure for separation and identification of selected catecholamines utiliaing high-performance and reversed-phase liquid chromatography is described. Levels of adrenal norepinephrine in spontanenouly hypertensive rats at 13 weeks of age were significantly (p<0.001) higher than those in age-matched normotensive Wistar Kyoto rats. Similar tendency was also noted for adrenal epinephrine to a lesser extent (p<0.01). The content of adrenal dopamine was, however, similar in both rat strains.