1983 Volume 74 Issue 8 Pages 1297-1312
In the present study, the authors tried to establish transplantable urogenital carcinomas onto nude mice and evaluate anticancer drugs using these experimental models.
The results were as follows:
Urogenital carcinomas, which were obtained from sixty-one patients during the surgical treatments, were aseptically xenotransplanted onto nude mice. The primary take developed in thirteen cases (21.3%). These included three cases of renal call caricinoma (21.4%), three of renal pelvis or bladder carcinoma (30.0%) and seven of testicular tumor (43.8%). The primary take did not developed in to prostatic carcinoma.
Of these thirteen cases with primary take, eight were established as the transplantable tumors (13.1%). They were two cases of renal cell carcinoma (14.2%), two of renal pelvis or bladder carcinoma (20.0%) and four of testicular tumor (25.0%). The transplantable tumors preserved all histological characteristics observed in their original tumors.
To transplantable tumors of renal cell carcinoma passed before four weeks, CDDP (5.0mg/kg) was administrated by intraperitoneal injection once in every two weeks. No difference, however, was shown in the estimated tumor weight between the CDDP group and the control (p>0.1).
In transplantable tumors of embryonal carcinoma of testis passed before two weeks of experiment, an intraperitoneal injection of CDDP (3.0mg/kg) once a week for four weeks revealed a significant difference in the estimated tumor weight as compared to that of the control group (p<0.01). No significant reduction in the estimated tumor weight was shown in the groups with BLM (5.0, 10, 20mg/kg) and with VBL (0.5, 1.0, 2.0mg/kg) (p>0.1). Intraperitoneal injection with a combination of BLM (10mg/kg) and VBL (1.0mg/kg) also showed the same results (p>0.05).
EMP (100, 150mg/kg), which was administrated by intraperitoneal injection twice a week for three weeks, did not cause reduction in the estimated tumor weight in transplantable tumors of prostatic carcinoma passed before six weeks. Intraperitoneal injection with a combination of CPM (20mg/kg), 5FU (30mg/kg) and MMC (0.1mg/kg) showed the same result as those for EMP.
The results in the experiments described above are parallel to those in experiences with clinical patients with renal cell carcinoma, testicular tumor and prostatic carcinoma. Therefore, transplantable urogenital carcinoma on nude mice is recommended as an experimental model for the screening of anticancer drugs.
