1983 Volume 74 Issue 8 Pages 1313-1319
Adjuvant immunization of rabbits was performed by the routine methods using γ-Sm and β-MSP separated and purified from seminal plasma by the previously reported method to prepare anti γ-Sm and anti β-MSP serum.
Localization of γ-Sm and β-MSP was examined using these anti-sera by the enzyme antibody method (PAP method: Peroxidase-Antiperoxidase method) in normal tissue and primary prostatic cancer.
Prostatic glandular epithelial cells, reserve cells and prostatic glandular secretions were stained specifically by the enzyme antibody method in normal and hypertrophic prostatic glands, but no specific staining was noted in the seminal vesicle spermatic duct and testis.
Specific staining was noted in primary prostatic cancer and metastatic prostatic cancer.
By the enzyme antibody method, we recognized that γ-Sm and β-MSP were prostatic specific antigens.