1984 Volume 75 Issue 6 Pages 945-950
The DNA synthesis in mixed lymphocyte culture reaction was measured by flow cytometry. The rate of lymphocyte blastogenesis was calculated from the DNA histogram as percent dividing compartment as follows.
The cells in the S+G2+M phases of the cell cycle were regarded as belonging to the dividing compartment. Percent dividing compartment was calculated by dividing the number of cells present outside the G0/G1 phase (S+G2+M) by the half number of cells (responder cells) in the histogram.
We compared percent dividing compartment with usual stimulation index and they showed good correlation (r=0.9493, p<0.01, n=12).
Therefore, this method seems to be a useful histocompatibility test without using radioisotopes instead of ordinary MLR.
