1985 Volume 76 Issue 2 Pages 165-173
BALB/c mice were immunized with a purified specific antigenic substance designated as γ-Seminoprotein (γ-Sm).
γ-Sm monoclonal antibodies (GS-2 antibodies) were generated by fusing cells from BALB/c mice immunized with the mouse myeloma cell line (NSL-cell line). We prepared the enzymed linked immunoabsorbant assay (ELISA).
And we attempted to establish diagnotic and progrotic usefulness of these protate specific antigens, γ-Sm, in serum of patients by this assay.
γ-Sm in serum were measured by ELISA in 38 patients with histologically verified untreated prostatic cancer, 75 with treated cancer, 48 with prostatic hypertrophy and 13 with other malignancy.
The mean of γ-Sm levels of 71 normal male, aged 10 to 70 was 1.78ng/ml and an-upper-limit of normal γ-Sm levels was 11.8ng/ml.
All patients with prostatic carcinoma showed elevated γ-Sm higher than 11.8ng/ml and clinical evaluation revealed that detection of early prostatic cancer with γ-Sm is a limited one.
